anti ema 1 (Developmental Studies Hybridoma Bank)
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Developmental Studies Hybridoma Bank
anti ema 1
Anti Ema 1, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 94/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+ema+1/anti-primordial+germ+cell+surface+marker/pmc13000516-99-17-23
Average 94 stars, based on 26 article reviews
Anti Ema 1, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 94/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+ema+1/anti-primordial+germ+cell+surface+marker/pmc13000516-99-17-23
Average 94 stars, based on 26 article reviews
anti ema 1 - by Bioz Stars,
2026-09
94/100 stars
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Incubation:Article Title: PGC-mediated conservation strategies for germplasm resources of Rugao Yellow Chicken and Shouguang Chicken in China1 Article Snippet: After washing the PGCs three times with PBS, they were fixed in 4% paraformaldehyde (Solarbio, China) for 30 min at room temperature on the slide until the cells attached to the slide, then washed twice with PBS. .. Next, the PGCs were permeabilized with PBS containing 0.5% Triton X-100 (Solarbio, China) at room temperature for 15 min and incubated in blocking solution (PBS containing 1.0% BSA) at room temperature for 1 h. The PGCs were then incubated overnight at 4°C with primary antibody anti-SSEA-1 (1:100; MC-480, DSHB, Lowa, USA), Article Title: Biochemical components of chicken primordial germ cells are modified by cryopreservation: original analysis by Fourier Transform Infrared (FTIR) microspectroscopy Article Snippet: .. For extracellular markers, slides were incubated overnight at +5°C with mouse anti-SSEA-1 (MC-480) at 1:65 dilution or Article Title: Comparative study of PGCs cultivation systems HiS and FAcs: a transcriptomic and cellular biology perspective Article Snippet: Subsequently, PGCs were permeabilized with PBS containing 0.5% Triton X-100 (Solarbio, Beijing, China) for 15 min at room temperature and then blocked in PBS containing 1.0% BSA for 1 h at room temperature. .. Overnight, PGCs were incubated at 4°C with primary antibodies, including anti-SSEA-1 (1:100; MC-480, DSHB, Iowa), Article Title: Influence and Optimization of Diverse Culture Systems on Chicken Embryonic Stem Cell Culture Article Snippet: ESC-like clones were washed three times with PBS, fixed with 4% paraformaldehyde (Solarbio, China) for 30 min at room temperature, and washed twice with PBS. .. Cells were then permeabilized using PBS with 0.5% Triton X-100 (Solarbio, China) for 15 min at room temperature and blocked in PBS containing 1.0% BSA for 1 h. The primary antibodies—anti-SSEA-1 (1:100; MC-480, DSHB, IA, USA), Article Title: Biochemical components of chicken primordial germ cells are modified by cryopreservation: original analysis by Fourier Transform Infrared (FTIR) microspectroscopy Article Snippet: .. For extracellular markers, slides were incubated overnight at +5 °C with mouse anti-SSEA-1 (MC-480) at 1:65 dilution or Article Title: Clonally derived chicken primordial germ cell lines maintain biological characteristics and proliferative potential in long-term culture. Article Snippet: Chicken primordial germ cells (PGCs) are important cells with significant implications in preserving genetic resources, chicken breeding and production, and basic research on genetics and development.. Currently, chicken PGCs can be cultured long-term in vitro to produce single-cell clones.. However, systematic exploration of the cellular characteristics of these single-cell clonal lines has yet to be conducted. Blocking Assay:Article Title: PGC-mediated conservation strategies for germplasm resources of Rugao Yellow Chicken and Shouguang Chicken in China1 Article Snippet: After washing the PGCs three times with PBS, they were fixed in 4% paraformaldehyde (Solarbio, China) for 30 min at room temperature on the slide until the cells attached to the slide, then washed twice with PBS. .. Next, the PGCs were permeabilized with PBS containing 0.5% Triton X-100 (Solarbio, China) at room temperature for 15 min and incubated in blocking solution (PBS containing 1.0% BSA) at room temperature for 1 h. The PGCs were then incubated overnight at 4°C with primary antibody anti-SSEA-1 (1:100; MC-480, DSHB, Lowa, USA), Article Title: Comparative study of PGCs cultivation systems HiS and FAcs: a transcriptomic and cellular biology perspective Article Snippet: Subsequently, PGCs were permeabilized with PBS containing 0.5% Triton X-100 (Solarbio, Beijing, China) for 15 min at room temperature and then blocked in PBS containing 1.0% BSA for 1 h at room temperature. .. Overnight, PGCs were incubated at 4°C with primary antibodies, including anti-SSEA-1 (1:100; MC-480, DSHB, Iowa), Article Title: Influence and Optimization of Diverse Culture Systems on Chicken Embryonic Stem Cell Culture Article Snippet: ESC-like clones were washed three times with PBS, fixed with 4% paraformaldehyde (Solarbio, China) for 30 min at room temperature, and washed twice with PBS. .. Cells were then permeabilized using PBS with 0.5% Triton X-100 (Solarbio, China) for 15 min at room temperature and blocked in PBS containing 1.0% BSA for 1 h. The primary antibodies—anti-SSEA-1 (1:100; MC-480, DSHB, IA, USA), Article Title: Clonally derived chicken primordial germ cell lines maintain biological characteristics and proliferative potential in long-term culture. Article Snippet: Chicken primordial germ cells (PGCs) are important cells with significant implications in preserving genetic resources, chicken breeding and production, and basic research on genetics and development.. Currently, chicken PGCs can be cultured long-term in vitro to produce single-cell clones.. However, systematic exploration of the cellular characteristics of these single-cell clonal lines has yet to be conducted. |